Deep mutational scanning highlights a role for cytosolic regions in Hrd1 function.
Peterson BG, Hwang J, Russ JE, Schroeder JW, Freddolino PL, Baldridge RD
Cell Reports, 2023, 42 (11), 113451
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Everyone has tried to fit pieces of something they've broken back together to make it work again. This is one approach we use in our everyday science
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Liposomes!
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Beautiful.
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Toothless... the workhorse of our lab...
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Our favorite protein quality control system.
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Proteoliposomes float to the top!
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This is home for our yeasty-beasties
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Our model organism of choice.
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Proteoliposomes imaged with a single-molecule setup
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mScarlet-I purification
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View from the lab
Protein Quality Control at Cellular Membranes
Our lab focuses on the mechanisms that cells use to recognize and dispose of misfolded proteins within their organelles. These processes are remarkably conserved among eukaryotes and we use Saccharomyces cerevisiae as a model system because of its simplicity relative to "higher" eukaryotes.Selected Publications
View all »Direct observation of autoubiquitination for an integral membrane ubiquitin ligase in ERAD
Moochickal Assainar B, Ragunathan K, Baldridge RD
BioRxiv : the Preprint Server for Biology, 2023
View on PubMed »Identification of ERAD-dependent degrons for the endoplasmic reticulum lumen
Sharninghausen R, Hwang J, Dennison D, Baldridge RD
BioRxiv : the Preprint Server for Biology, 2023
View on PubMed »The ERAD system is restricted by elevated ceramides.
Hwang J, Peterson BG, Knupp J, Baldridge RD
Science Advances, 2023, 9 (2), eadd8579
View on PubMed »Cycles of autoubiquitination and deubiquitination regulate the ERAD ubiquitin ligase Hrd1.
Peterson BG, Glaser ML, Rapoport TA, Baldridge RD
eLife, 2019, 8
View on PubMed »Autoubiquitination of the Hrd1 ligase triggers protein retrotranslocation in ERAD.
Baldridge RD, Rapoport TA
Cell, 2016, 166 (2), 394-407
View on PubMed »Latest News
View all »He did it!
Congratulations to Dr. Brian Peterson! The first PhD from the lab!
Basila’s preprint is available!
Congratulations on this elegant reconstitution – in collaboration with her joint mentor Kaushik Ragunathan (@Brandeis University) https://www.biorxiv.org/content/10.1101/2023.06.20.545802v1
Rachel+Jiwon and Devon’s preprint is available!
This is an exciting discovery reporting on the first ER-localized degrons. (https://www.biorxiv.org/content/10.1101/2023.06.21.546000v1)
Brian’s (and Jiwon+Jenn) preprint is out!
This is an amazing body of work congratulations! https://www.biorxiv.org/content/10.1101/2023.04.03.535444v1
Congratulations to Evan!
Evan earned his MSc in Biological Chemistry.
Jiwon’s publication is out! Congratulations to her and Brian!
https://www.science.org/doi/10.1126/sciadv.add8579
Too many things stuck together (v3… Jenn and Jeremy were both selected for NIH training grants!)
Jenn Russ was selected for the GTP (a highly competitive Genetics Training Program T32 training grant)!
Jeremy Dortch was selected for the PSTP (a highly competitive Pharmacological Sciences Training Program T32 training grant)!
An important honor for amazing students.
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