Everyone has tried to fit pieces of something they've broken back together to make it work again. This is one approach we use in our everyday science
Extruding liposomes!
Our favorite protein quality control system (probably).
Pretty protein purifications
Toothless... the workhorse of our lab...
Proteoliposomes float near the top!
This is home for our yeasty-beasties
Proteoliposomes imaged with a single-molecule setup
mScarlet-I purification
View from the lab
Yeast as a model organism.
Competition between Der1 and ERAD-M substrates controls Hrd1 complex function.
Russ JE*, Peterson BG*, Taylor S, Baldridge RD
Proceedings of the National Academy of Sciences of the United States of America, 2025, 122 (49), e2513595122
View on PubMed »Ubiquitin chain variability directs substrates of the Tul1 ubiquitin ligase complex to different degradation pathways.
Dennison DD, Baldridge RD
The Journal of Cell Biology, 2025, 224 (9)
View on PubMed »Identification of ERAD-dependent degrons for the endoplasmic reticulum lumen.
Sharninghausen R*, Hwang J*, Dennison DD, Baldridge RD
eLife, 2024, 12
View More »Direct observation of autoubiquitination for an integral membrane ubiquitin ligase in ERAD.
Moochickal Assainar B, Ragunathan K*, Baldridge RD*
Nature Communications, 2024, 15 (1), 1340
View More »Deep mutational scanning highlights a role for cytosolic regions in Hrd1 function.
Peterson BG, Hwang J, Russ JE, Schroeder JW, Freddolino PL, Baldridge RD
Cell Reports, 2023, 42 (11), 113451
View More »The ERAD system is restricted by elevated ceramides.
Hwang J, Peterson BG, Knupp J, Baldridge RD
Science Advances, 2023, 9 (2), eadd8579
View More »Cycles of autoubiquitination and deubiquitination regulate the ERAD ubiquitin ligase Hrd1.
Peterson BG, Glaser ML, Rapoport TA, Baldridge RD
eLife, 2019, 8
View More »Autoubiquitination of the Hrd1 ligase triggers protein retrotranslocation in ERAD.
Baldridge RD, Rapoport TA
Cell, 2016, 166 (2), 394-407
View on PubMed »The Baldridge Lab and Tsai lab received a grant from the W. M. Keck Foundation to develop novel targeted protein degradation strategies for targeting proteins from the ER to inhibit viral biogenesis and infection.
Congratulation to Dr. Devon Dennison! She earned the second PhD from the lab with her thesis titled: “Exploring the Mechanics of Golgi-localized Tul1 Selective Degradation.”
This is really an amazing body of work that was helped across the finish line by Jiwon Hwang and Jenn Russ, along with our collaborators Jeremy Schroeder and Lydia Freddolino… Congratulations!
Final article at Cell Reports: https://doi.org/10.1016/j.celrep.2023.113451
bioRxiv preprint: https://www.biorxiv.org/content/10.1101/2023.04.03.535444v1
Congratulations to Dr. Brian Peterson! The first PhD from the lab!
Congratulations on this elegant reconstitution – in collaboration with her joint mentor Kaushik Ragunathan (@Brandeis University) https://www.biorxiv.org/content/10.1101/2023.06.20.545802v1
This is an exciting discovery reporting on the first ER-localized degrons. (https://www.biorxiv.org/content/10.1101/2023.06.21.546000v1)
https://www.science.org/doi/10.1126/sciadv.add8579
